標(biāo)題：山萘酚靶向脂質(zhì)體的制備與評(píng)價(jià)

鏈接：https://xueshu.baidu.com/usercenter/paper/show?paperid=7798a3dbd8f6faa67daba37ba632887f&site=xueshu_se

作者：劉祖浩，雷玉杰，宋浩穎，常聰

摘要：

We have developed a new type of PEG-immunoliposome carrying monoclonal antibodies or their fragments (F(ab′)2, Fab′) at the distal ends of the PEG chains (Type C). Distearoylphosphatidylethanolamine derivatives of PEG with car?yl group (DSPE-PEG-COOH) or dipalmitoyl phosphatidylethanolamine derivatives of PEG with maleimidyl group (DPPE-PEG-Mal) at the PEG terminal were newly synthesized. Small unilamellar liposomes (90–130 nm in diameter) were prepared from distearoyl phosphatidylcholine and cholesterol (2:1, m/m) containing 6 mol% of DSPE-PEG-COOH or DPPE-PEG-Mal. To target to the vascular endothelial lung surface as a model accessible site, 34A antibody, which is highly specific to mouse pulmonary endothelial cells, was conjugated to PEG-liposome (34A-Type C). The degree of lung binding of 34A-Type C in BALB/c mice was significantly higher than that of the 34A-Type A which is an ordinary type immunoliposome (without PEG derivatives).

譯文：

我們開發(fā)了一種新型的PEG免疫脂質(zhì)體，在PEG鏈的遠(yuǎn)端攜帶單克隆*體或其片段（F（ab′）2，F(xiàn)ab′）（C型）。聚乙二醇二硬脂酰磷脂酰乙醇胺衍生物?新合成了PEG末端帶有馬來酰亞胺基的烷基（DSPE-PEG-COOH）或二棕櫚酰磷脂酰乙醇胺衍生物（DPPE-PEG-Mal）。由含有6mol%DSPE-PEG-COOH或DPPE-PEG-Mal的二硬脂酰磷脂酰膽堿和膽固醇（2:1，m/m）制備了小單層脂質(zhì)體（直徑90-130nm）。為了靶向血管內(nèi)皮肺表面作為模型可及位點(diǎn)，將對(duì)小鼠肺內(nèi)皮細(xì)胞高度特異性的34A*體與PEG脂質(zhì)體（34A C型）結(jié)合。BALB/C小鼠中34A C型的肺結(jié)合程度明顯高于普通型免疫脂質(zhì)體（不含PEG衍生物）34A A型。

DOI：10.3969/j.issn.1008-987x.2021.03.09

西安pg電子官方生物提供相關(guān)產(chǎn)品：

DSPE-PEG-acid

DSPE-PEG4-acid

DSPE-PEG-Ald

DSPE-PEG-Amine，1069-79-0

DSPE-PEG-azide

DSPE-PEG5-azide

DSPE-PEG-Biotin

DSPE-PEG-Boronate

DSPE-PEG-CH2COOH

以上文章內(nèi)容轉(zhuǎn)載自網(wǎng)絡(luò)，如有侵權(quán)請(qǐng)聯(lián)系我們刪除！