去甲斑蝥素DSPE-PEG-MAL納米粒的制備

文獻(xiàn)鏈接：https://xueshu.baidu.com/usercenter/paper/show?paperid=1u7y0gj0ry6b0880de3f0ac0s6129880&site=xueshu_se

文獻(xiàn)作者：JS Cudnik，PC West，RK Fisher，Sam Mattern-Schain，M Best，SS Kirkpatrick，MB Freeman，OH Grandas，DJ Mountain

目的:制備去甲斑蝥素DSPE-PEG-MAL納米粒,探討納米粒接上碳酸酐酶Ⅸ*體后的*腫瘤作用變化. 

方法:將碳酸酐酶Ⅸ*體孵化修飾去甲斑蝥素DSPE-PEG-MAL納米粒,考察其載藥率,包封率,粒徑等指標(biāo);通過(guò)MTT法考察去甲斑蝥素及載藥納米粒對(duì)A549的細(xì)胞生存率,為細(xì)胞攝取實(shí)驗(yàn)劑量設(shè)置提供依據(jù);取荷瘤裸鼠分別尾靜脈注射去甲斑蝥素和載藥納米粒,并觀察裸鼠的飲食,精神狀態(tài),每周用游標(biāo)卡尺測(cè)量腫瘤的大小,繪制腫瘤生長(zhǎng)曲線. 

結(jié)果:制備的碳酸酐酶Ⅸ*體修飾的去甲斑蝥素納米粒呈圓球狀,載藥量為(5.26±0.03)%,包封率為(80.93±1.01)%,粒徑和Zeta電位分別為146.5±48.9nm,-(14.79±0.67)mv;去甲斑蝥素及載藥納米粒對(duì)腫瘤細(xì)胞的抑制作用呈劑量和時(shí)間依賴性,載藥納米粒對(duì)腫瘤細(xì)胞的抑制作用明顯優(yōu)于游離藥物,且經(jīng)碳酸酐酶Ⅸ*體修飾后納米粒對(duì)A549的抑制率加強(qiáng). 結(jié)論:去甲斑蝥素納米粒經(jīng)碳酸酐酶Ⅸ*體修飾后能達(dá)到主動(dòng)靶向的效果,同時(shí)能與去甲斑蝥素協(xié)同抑瘤,起到一舉多得的目的.

譯文：

Objective: To prepare DSPE-PEG-MAL nanoparticles of norcantharidin and investigate the changes in anti-tumor activity of the nanoparticles after attaching carbonic anhydrase IX antibody  

Method: Carbonic anhydrase IX antibody was incubated and modified with norcantharidin DSPE-PEG-MAL nanoparticles to investigate their drug loading rate, encapsulation efficiency, particle size, and other indicators; Using MTT assay to investigate the cell survival rate of A549 cells treated with norcantharidin and drug loaded nanoparticles, providing a basis for setting the experimental dose for cell uptake; Tumor bearing nude mice were injected with norcantharidin and drug loaded nanoparticles via tail vein, and their diet and mental state were observed. The size of the tumor was measured weekly using a vernier caliper, and the tumor growth curve was plotted  

Result: The prepared carbonic anhydrase IX antibody modified norcantharidin nanoparticles were spherical in shape, with a drug loading of (5.26 ± 0.03)% and an encapsulation efficiency of (80.93 ± 1.01)%. The particle size and Zeta potential were 146.5 ± 48.9nm and - (14.79 ± 0.67) mv, respectively. The inhibitory effects of norcantharidin and drug loaded nanoparticles on tumor cells were dose-dependent and time-dependent, and the inhibitory effect of drug loaded nanoparticles on tumor cells was significantly better than that of the free drug. Moreover, the inhibitory rate of nanoparticles on A549 was enhanced after modification with carbonic anhydrase IX antibody Conclusion: Norcantharidin nanoparticles can achieve active targeting after modification with carbonic anhydrase IX antibody, and can synergistically inhibit tumors with Norcantharidin, achieving a win-win effect

DOI：10.3969/j.issn.1008-987x.2021.03.09

西安pg電子官方生物提供相關(guān)產(chǎn)品：

DSPE-PEG-Mal

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DSPE-PEG-OH

DSPE-PEG-propargyl

DSPE-PEG-Alkyne

DSPE-PEG-Rhodamine

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DSPE-SPDP

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