文獻(xiàn)：Elucidating Driving Forces for Liposome Rupture: External Perturbations and Chemical Affinity

文獻(xiàn)鏈接：https://pubs.acs.org/doi/abs/10.1021/la300127m

作者：Xi WangMatthew M. ShindelSzu-Wen WangRegina Ragan

摘要：

Atomic force microscopy (AFM) studies under aqueous buffer probed the role of chemical affinity between liposomes, consisting of large unilamellar vesicles, and substrate surfaces in driving vesicle rupture and tethered lipid bilayer membrane (tLBM) formation on Au surfaces. 1,2-Distearoyl-sn-glycero-3-phosphoethanolamine-N-poly(ethylene glycol)-2000-N-[3-(2-pyridyldithio) propionate] (DSPE-PEG-PDP) was added to 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) vesicles to promote interactions via Au–thiolate bond formation. Forces induced by an AFM tip leading to vesicle rupture on Au were quantified as a function of DSPE-PEG-PDP composition with and without osmotic pressure. The critical forces needed to initiate rupture of vesicles with 2.5, 5, and 10 mol % DSPE-PEG-PDP are approximately 1.1, 0.8, and 0.5 nN, respectively. The critical force needed for tLBM formation decreases from 1.1 nN (without osmotic pressure) to 0.6 nN (with an osmotic pressure due to 5 mM of CaCl2) for vesicles having 2.5 mol % DSPE-PEG-PDP. Forces as high as 5 nN did not lead to LBM formation from pure POPC vesicles on Au. DSPE-PEG-PDP appears to be important to anchor and deform vesicles on Au surfaces. This study demonstrates how functional lipids can be used to tune vesicle–surface interactions and elucidates the role of vesicle–substrate interactions in vesicle rupture.

在水性緩沖液下進(jìn)行的原子力顯微鏡（AFM）研究探討了由大型單層囊泡組成的脂質(zhì)體與基底表面之間的化學(xué)親和力在驅(qū)動(dòng)囊泡破裂和Au表面形成栓系脂質(zhì)雙層膜（tLBM）中的作用。

將1,2-二硬脂酰-sn-甘油-3-磷酸乙醇胺-N-聚（乙二醇）-2000-N-[3-（2-吡啶基二硫代）丙酸酯]（DSPE-PEG-PDP）加入1-棕櫚酰-2-油�；�-sn-甘油3-磷酸膽堿（POPC）囊泡中，以促進(jìn)通過金-硫醇鍵形成的相互作用。在有和沒有滲透壓的情況下，AFM尖端引起的導(dǎo)致Au上囊泡破裂的力被量化為DSPE-PEG-PDP成分的函數(shù)。

引發(fā)2.5、5和10 mol%DSPE-PEG-PDP囊泡破裂所需的臨界力分別約為1.1、0.8和0.5 nN。對(duì)于具有2.5mol%DSPE-PEG-PDP的囊泡，tLBM形成所需的臨界力從1.1nN（無滲透壓）降低到0.6nN（有5mM CaCl2的滲透壓）。

高達(dá)5nN的力不會(huì)導(dǎo)致Au上純POPC囊泡形成LBM。DSPE-PEG-PDP似乎對(duì)Au表面上的囊泡錨定和變形很重要。本研究展示了功能性脂質(zhì)如何用于調(diào)節(jié)囊泡-表面相互作用，并闡明了囊泡-底物相互作用在囊泡破裂中的作用。

相關(guān)推薦：

DSPE-PEG-PDP

DSPE-PEG-PEI

DSPE-PEG-PLA

DSPE-PEG-PLGA

DSPE-PEG-Progestrone

DSPE-PEG-RGD

DSPE-PEG-SC

DSPE-PEG-SCM

DSPE-PEG-SG

DSPE-PEG-SH

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