文獻(xiàn)：Self-assembled liposome-loaded microbubbles: The missing link for safe and efficient ultrasound triggered drug-delivery

文獻(xiàn)鏈接：https://www.sciencedirect.com/science/article/abs/pii/S016836591100109X

作者：Bart Geers a, Ine Lentacker a, Niek N. Sanders b, Joseph Demeester a, Stephen Meairs c, Stefaan C. De Smedt a

摘要：

Liposome-loaded microbubbles have been recently introduced as a promising drug delivery platform for ultrasound guided drug delivery. In this paper we design liposome-loaded (lipid-shelled) microbubbles through the simple self-assembly of the involved compounds in a single step process. We thoroughly characterized the liposome-loading of the microbubbles and evaluated the cell killing efficiency of this material using doxorubicin (DOX) as a model drug. Importantly, we observed that the DOX liposome-loaded microbubbles allowed killing of melanoma cells even at very low doses of DOX. These findings clearly prove the potential of liposome-loaded microbubbles for ultrasound targeted drug delivery to cancer tissues.

功能化微泡的制備與表征

“功能化微泡”（即含有巰基功能化PEG脂質(zhì)的微泡）是從1,2-二棕櫚�；�-sn-甘油-3-磷酸膽堿（DPPC）和1,2-二硬脂酰基-sn-丙三醇胺-N-[PDP（聚乙二醇）-2000]（DSPE-PEG-SPDP）（Avanti極性脂質(zhì)，阿爾拜斯特，AL）在1:2:7甘油-丙二醇-H2O溶劑（Sigma aldrich，Bornem，Belgium）中的脂質(zhì)溶液開(kāi)始制備的，脂質(zhì)溶液中脂質(zhì)的摩爾比分別為

脂質(zhì)體自組裝微泡的制備

如圖1所示，為了制備負(fù)載脂質(zhì)體的微泡，向（官能化）脂質(zhì)DPPC和DSPE-PEG-SPDP的混合物中加入含有DSPE-PEG-MALEIMIDE的脂質(zhì)體，將其溶解在甘油-丙二醇-水溶劑中，濃度低于其臨界膠束濃度。

相關(guān)推薦：

DSPE-PEG-cRGD

DPPE-PEG-cRGD

DMPE-PEG-cRGD

DOPE-PEG-cRGD

DPPE-PEG-RGD

DMPE-PEG-RGD

DOPE-PEG-RGD

4-Arm PEG-DSPE

C12-PEG-COOH

版權(quán)聲明：

本平臺(tái)根據(jù)相關(guān)科技期刊文獻(xiàn)、教材以及網(wǎng)站編譯整理的內(nèi)容，僅用于對(duì)相關(guān)科學(xué)作品的介紹、評(píng)論以及課堂教學(xué)或科學(xué)研究。如有侵權(quán)，請(qǐng)聯(lián)系我們刪除。