文獻(xiàn)：Research on mechanism of curcumin carried with PLGA-DSPE-PEG hybridized nanoparticles on regulating the nuclear receptor coactivator5 presentation, oxidative stress and presentation of alfa-fetoprotein in rats with liver cancer

文獻(xiàn)鏈接：https://xueshu.baidu.com/usercenter/paper/show?paperid=1h0p0pr0ka6d0ma018430je0u1436527&site=xueshu_se

作者：作者：F Dai，W Chen，H Li

摘要：

This study intends to discuss the mechanism of curcumin carried with poly (lactic-co-glycolic acid)-1, 2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[amino (polyethylene glycol)-2000] (PLGA-DSPE-PEG) hybridized nanoparticles on regulating the Nuclear receptor coactivator5 (NCOA5) expression, oxidative stress and level of Alfa-fetoprotein (AFP) in rats with liver cancer. Curcumin carried with PLGA-DSPE-PEG hybridized nanoparticles was prepared. These rats were divided into normal set, model set, curcumin set and set of curcumin and nanoparticles randomly. The presentation of alanine transaminase (ALT) and aspartate aminotransferase (AST) in every set was detected. The pathological change in liver tissue with hematoxylin and eosin (H&E) staining method, level of malondialdehyde (MDA) and superoxide dismutase (SOD) was detected. Presentation of AFP and NCOA5 was detected with Western Blotting assay and real-time reverse transcription-polymerase chain reaction (RT-PCR). 

本研究旨在探討聚（丙交酯-共-乙醇酸）-1，2-二硬脂�；�-sn-甘油-3-磷酸乙酰胺-N-[氨基（聚乙二醇）-2000]（PLGA-DSPE-PEG）雜化納米顆粒攜帶的姜黃素對(duì)*癥大鼠核受體輔激活因子5（NCOA5）表達(dá)、氧化應(yīng)激和α-鐵蛋白（AFP）水平的調(diào)節(jié)作用機(jī)制。

制備了攜帶PLGA-DSPE-PEG雜化納米顆粒的姜黃素。這些大鼠被隨機(jī)分為正常組、模型組、姜黃素組和姜黃素和納米粒組。檢測(cè)每組中丙氨酸轉(zhuǎn)氨酶（ALT）和天冬氨酸轉(zhuǎn)氨酶（AST）的表現(xiàn)。

采用蘇木精伊紅（H&E）染色法檢測(cè)肝組織病理變化，丙二醛（MDA）和超氧化物歧化酶（SOD）水平。采用Western Blotting法和實(shí)時(shí)逆轉(zhuǎn)錄聚合酶鏈?zhǔn)椒磻?yīng)（RT-PCR）檢測(cè)AFP和NCOA5的表達(dá)。正常組ALT、AST、MDA和AFP水平最高，姜黃素和納米粒組最低。姜黃素和納米顆粒組中的SOD呈現(xiàn)率最高。在正常設(shè)置下，表面紋理清晰，光澤度好，沿邊緣排列有序。PLGA-DSPE-PEG雜交納米粒子攜帶的姜黃素可降低*癥大鼠的ALT和AST水平。SOD活性升高，MDA降低。AFP表達(dá)減少，NCOA5表達(dá)增加。  

相關(guān)推薦：

C18-PEGn-NHS

C18-PEGn-OH

C18-PEGn-OPSS

C18-PEGn-SH (SH: Thiol)

mPEG-Cholesterol (mPEG-CLS)

mPEG-CONH-C12

mPEG-CONH-C16

mPEG-CONH-C18

mPEG-DMPE

mPEG-DSPE

mPEG-O-C12

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