文獻(xiàn)：Design of pH-sensitive methotrexate prodrug-targeted curcumin nanoparticles for efficient dual-drug delivery and combination cancer therapy

文獻(xiàn)鏈接：https://www.tandfonline.com/doi/full/10.2147/IJN.S152312#d1e205

作者：Jiajiang Xie,Zhongxiong Fan,Yang Li,Yinying Zhang,Fei Yu,Guanghao Su

摘要：

To investigate the intracellular drug/nanocarrier delivery of MTX-Imine-M-CUR, Cy5.5-labeled DSPE-PEG was synthesized for use as a near-infrared fluorescent probe (DSPE-PEG-Cy5.5 was conjugated between DSPE-PEG-NH2 and Cy5.5 NHS ester via amide linkage). HeLa cells were seeded into 24-well plates at a density of 1.0×104 cells per well. After culturing for 24 h, the cells were treated with MTX-Imine-MCy5.5-CUR at 37°C for predetermined incubation time periods. Afterward, the cells were washed three times with PBS and fixed with 4% (v/v) paraformaldehyde for 15 min. Finally, the cells were washed by cold PBS twice, stained with DAPI for 10 min, and observed using a Leica TCS SP5 CLSM.

為了研究MTX-Imine-M-CUR的細(xì)胞內(nèi)藥物/納米載體遞送，合成了Cy5.5標(biāo)記的DSPE-PEG作為近紅外熒光探針（DSPE-PEG-Cy5.5通過酰胺鍵結(jié)合在DSPE-PEG-NH2和Cy5.5 NHS酯之間）。

HeLa細(xì)胞以每孔1.0×104個細(xì)胞的密度接種到24孔板中。培養(yǎng)24小時后，在37°C下用MTX-Imine-MCy5.5-CUR處理細(xì)胞預(yù)定的孵育時間。然后，用PBS洗滌細(xì)胞三次，用4%（v/v）多聚甲醛固定15分鐘。

最后，用冷PBS洗滌細(xì)胞兩次，用DAPI染色10分鐘，并使用Leica TCS SP5 CLSM觀察。

相關(guān)推薦產(chǎn)品：

Cy7-PEG-DPPE

DSPE-PEG-Cy7

DSPE-PEG-cRGD

DPPE-PEG-cRGD

DMPE-PEG-cRGD

DOPE-PEG-cRGD

DPPE-PEG-RGD

DMPE-PEG-RGD

DOPE-PEG-RGD

4-Arm PEG-DSPE

C12-PEG-COOH

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