DSPE-PEG-PDP調(diào)控金表面脂質(zhì)體破裂行為及tLBM形成機(jī)制研究

鏈接：https://pubs.acs.org/doi/abs/10.1021/la300127m

作者：王曦馬修·M·辛德爾王思文雷吉娜·拉根*

摘要：

在水性緩沖液條件下，利用原子力顯微鏡 (AFM) 研究了脂質(zhì)體（由大型單層囊泡組成）與基底表面之間的化學(xué)親和力，探究其在金表面驅(qū)動(dòng)囊泡破裂和束縛脂質(zhì)雙層膜 (tLBM) 形成的作用。將 1,2-二硬脂酰-sn-甘油-3-磷酸乙醇胺-N-聚乙二醇-2000 -N- [3-(2-吡啶基二硫代)丙酸酯] (DSPE-PEG-PDP) 添加到 1-棕櫚酰-2-油酰-sn-甘油-3-磷酸膽堿 (POPC) 囊泡中，以促進(jìn)通過金-硫醇鹽鍵形成的相互作用。在有滲透壓和無滲透壓條件下，AFM 探針探針誘導(dǎo)的導(dǎo)致金表面囊泡破裂的力被量化為 DSPE-PEG-PDP 組分的函數(shù)。引起含有 2.5、5 和 10 mol % DSPE-PEG-PDP 的囊泡破裂所需的臨界力分別約為 1.1、0.8 和 0.5 nN。對(duì)于含有 2.5 mol % DSPE-PEG-PDP 的囊泡，tLBM 形成所需的臨界力從 1.1 nN（無滲透壓）降至 0.6 nN（5 mM CaCl 2引起的滲透壓）。高達(dá) 5 nN 的力也不導(dǎo)致純 POPC 囊泡在金上形成 LBM。DSPE-PEG-PDP 似乎對(duì)于在金表面錨定和變形囊泡很重要。這項(xiàng)研究展示了如何利用功能性脂質(zhì)來調(diào)節(jié)囊泡-表面相互作用，并闡明了囊泡-底物相互作用在囊泡破裂中的作用。

Abstract

Atomic force microscopy (AFM) studies under aqueous buffer probed the role of chemical affinity between liposomes, consisting of large unilamellar vesicles, and substrate surfaces in driving vesicle rupture and tethered lipid bilayer membrane (tLBM) formation on Au surfaces. 1,2-Distearoyl-sn-glycero-3-phosphoethanolamine-N-poly(ethylene glycol)-2000-N-[3-(2-pyridyldithio) propionate] (DSPE-PEG-PDP) was added to 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) vesicles to promote interactions via Au–thiolate bond formation. Forces induced by an AFM tip leading to vesicle rupture on Au were quantified as a function of DSPE-PEG-PDP composition with and without osmotic pressure. The critical forces needed to initiate rupture of vesicles with 2.5, 5, and 10 mol % DSPE-PEG-PDP are approximately 1.1, 0.8, and 0.5 nN, respectively. The critical force needed for tLBM formation decreases from 1.1 nN (without osmotic pressure) to 0.6 nN (with an osmotic pressure due to 5 mM of CaCl2) for vesicles having 2.5 mol % DSPE-PEG-PDP. Forces as high as 5 nN did not lead to LBM formation from pure POPC vesicles on Au. DSPE-PEG-PDP appears to be important to anchor and deform vesicles on Au surfaces. This study demonstrates how functional lipids can be used to tune vesicle–surface interactions and elucidates the role of vesicle–substrate interactions in vesicle rupture.

西安pg電子官方生物提供相關(guān)產(chǎn)品：

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