文獻(xiàn)：表面活性蛋白A納米*體偶聯(lián)脂質(zhì)體負(fù)載甲潑尼龍?zhí)岣叻伟邢蛱禺愋约皩?duì)急性肺損傷的*效果

鏈接：https://www.tandfonline.com/doi/full/10.1080/10717544.2017.1402217#abstract

作者：李楠，東翁，王善梅，張?jiān)�，陳珊珊，尹兆芳，齋嘉麗，朱迪·斯科布爾，夏洛特·C·威廉姆斯，陳濤，邱惠，秦五，趙萌萌，盧立勤，澤維爾·穆萊特&李慧萍

材料

1,2-二硬脂酰-sn-甘油-3-磷酸膽堿 (DSPC)、硝基苯并呋咱 (DSPE-PEG-NBD) 購(gòu)自 Avanti Polar Lipids, Inc. (阿拉巴馬州阿拉巴斯特)。1,2-二硬脂酰-sn-甘油-3-磷酸乙醇胺-PEG 3400 -NH 2 (DSPE-PEG 3400 -NH 2 ) 購(gòu)自 Nanocs Inc. (紐約州紐約市)。N-琥珀酰亞胺碘乙酸酯 (SIA 交聯(lián)劑) 購(gòu)自 Thermo Scientific (馬薩諸塞州沃爾瑟姆)。磷酸鹽緩沖液 (PBS, pH 7.4) 和膽固醇 (CHOL) 購(gòu)自 Sigma-Aldrich (密蘇里州圣路易斯)。SP-A *原由上海優(yōu)龍生物科技有限公司 (中國(guó)上海) 合成。 SP-A納米*體（SPANb）由上海市肺科醫(yī)院呼吸疾病實(shí)驗(yàn)室合成（Gajic等，引文2011）。所有其他化學(xué)品和溶劑均為分析純。所有水相制備均使用Milli-Q H 2 O (18.2 MΩ)。所有化合物均未經(jīng)進(jìn)一步純化使用。

Materials

1,2-Distearoyl-sn-glycero-3-phosphocholine (DSPC), nitrobenzofurazan (DSPE-PEG-NBD) was purchased from Avanti Polar Lipids, Inc. (Alabaster, AL). 1,2-Distearoyl-sn-glycero-3-phosphoethanolamine-PEG3400-NH2 (DSPE-PEG3400-NH2) was purchased from Nanocs Inc. (New York, NY). N-Succinimidyl iodoacetate (SIA crosslinker) was purchased from Thermo Scientific (Waltham, MA). Phosphate buffered saline (PBS, pH 7.4) and cholesterol (CHOL) were purchased from Sigma-Aldrich (St. Louis, MO). SP-A antigen was synthesized by Shanghai YouLong Biotech Co., Ltd. (Shanghai, China). SP-A nanobody (SPANb) was synthesized by the Laboratory of Respiratory Disease, Shanghai Pulmonary Hospital, China (Gajic et al., Citation2011). All other chemicals and solvents were of analytical grade. Milli-Q H2O (18.2 MΩ) was used for all aqueous preparations. All compounds were used without further purification.

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