文獻(xiàn)：表面活性蛋白A納米*體偶聯(lián)脂質(zhì)體負(fù)載甲潑尼龍?zhí)岣叻伟邢蛱禺愋约皩?duì)急性肺損傷的*效果

鏈接：https://www.tandfonline.com/doi/full/10.1080/10717544.2017.1402217#abstract

作者：李楠，東翁，王善梅，張?jiān)�，陳珊珊，尹兆芳，齋嘉麗，朱迪·斯科布爾，夏洛特·C·威廉姆斯，陳濤，邱惠，秦五，趙萌萌，盧立勤，澤維爾·穆萊特&李慧萍

節(jié)選：

將 SPANb 與 MPS–NSSLs 共軛形成 MPS–NSSLs–SPANb

將SPANb與MPS-NSSLs混合，然后在4°C下孵育過夜以進(jìn)行結(jié)合。為了優(yōu)化結(jié)合效率，將SPANb和MPS-NSSL以不同的His標(biāo)簽與碘乙酸鹽的摩爾比混合。在XCell SureLock Mini-Cell電泳系統(tǒng)（Invitrogen，卡爾斯巴德，加利福尼亞州）上進(jìn)行十二烷基硫酸鈉聚丙烯酰胺凝膠電泳（SDS-PAGE）分析，以確認(rèn)SPANb與MPS-NSSLs的結(jié)合。使用Image J軟件（Bethesda，MD）對(duì)染色的凝膠進(jìn)行積分，計(jì)算結(jié)合效率百分比（Gajic等人，引文2011）。結(jié)合后，MPS–NSSLs–SPANb（SPANb 和 MPS-NSSLs 的混合物）通過凝膠過濾純化，純化柱為 Superose 12 10/300 柱（GE Healthcare，Chicago，IL），并用 PBS 平衡以去除游離的 SPANb。對(duì)于 FITC 標(biāo)記的 MPS-NSSLs-SPANb（MPS-NSSLs-SPANb-FITC），使用相同的方法，將 SPANb 替換為 FITC 標(biāo)記的 SPANb（SPANb-FITC）。使用 DSPE-PEG-NBD 替換/混合 MPS，然后使用相同方法制備 MPS-NSSLs-NBD/NSSLs-NBD。

Conjugating SPANb to MPS–NSSLs to form MPS–NSSLs–SPANb

SPANb was mixed with MPS–NSSLs and then incubated at 4?°C overnight for conjugation. SPANb and MPS–NSSL were mixed in different mole ratios of His-tag to iodoacetate to optimize conjugation efficiency. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) analysis on an XCell SureLock Mini-Cell Electrophoresis System (Invitrogen, Carlsbad, CA) was performed to confirm the conjugation of SPANb to MPS–NSSLs. Conjugation efficiency in percentage was calculated by integrating the stained gels using Image J software (Bethesda, MD) (Gajic et al., Citation2011). After conjugation, MPS–NSSLs–SPANb (the mixture of SPANb and MPS-NSSLs) was purified by gel filtration on a Superose 12 10/300 column (GE Healthcare, Chicago, IL) equilibrated with PBS to remove of the free SPANb. For the preparation of FITC-labeled MPS-NSSLs-SPANb (MPS-NSSLs-SPANb-FITC), the same protocol was used by replacing SPANb with FITC labeled SPANb (SPANb–FITC). DSPE-PEG-NBD was used to replace/mixed with MPS, and then MPS-NSSLs-NBD/NSSLs-NBD was prepared using the same method.

西安pg電子官方生物提供相關(guān)產(chǎn)品：

GE11-PEG-DSPE

PTP-PEG-DSPE

SP94-PEG-DSPE

T7(HAIYPRH)-PEG-DSPE

Octreotide-PEG-DSPE

YIGSR-PEG-DSPE

APRPG-PEG-DSPE

NGR-PEG-DSPE

NGR(c(GGCNGRC))-PEG-DSPE

RVG29-PEG-DSPE

THRPPMWSPVWP-PEG-DSPE

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