文獻(xiàn)：線粒體、溶酶體和內(nèi)質(zhì)網(wǎng)：哪個(gè)是光療的最佳靶點(diǎn)？

鏈接：https://www.sciencedirect.com/science/article/abs/pii/S0168365922006320

作者：李艷紅 ，賈浩然 ，王洪 銀，華先武 ，鮑彥文 ，吳福 根

節(jié)選：

DSPE-PEG-Ce6、甲氧基（OMe）-PEG 5000 -Ce6（OMe-PEG-Ce6）和DSPE-PEG 5000-異硫氰酸熒光素（FITC）（DSPE-PEG-FITC）的合成

合成DSPE-PEG-Ce6：將1.60 mg Ce6溶于1 mL二甲基亞砜（DMSO）中。然后，將4.16 mg 1-乙基-3-（3-二甲基氨基丙基）碳二亞胺（EDC）和3.09 mg  N-羥基琥珀酰亞胺（NHS）加入上述Ce6溶液中，室溫反應(yīng)4 h。接著，將活化后的Ce6加入5.17 mg DSPE-PEG 5000 -NH 2（預(yù)溶于DMSO）中，繼續(xù)攪拌反應(yīng)過夜。將混合物用DMSO透析（截留分子量（MWCO）= 2 kDa）

DSPE-PEG-Ce6膠束的合成與表征

本設(shè)計(jì)采用DSPE-PEG 5000 -NH 2作為藥物載體，因?yàn)檩^長(zhǎng)的PEG鏈可以作為阻隔生物成分的保護(hù)屏障，從而延長(zhǎng)藥物的循環(huán)時(shí)間[30,31]。Ce6是第二代多聚苯乙烯，可通過其羧基輕松修飾進(jìn)行化學(xué)結(jié)合。本設(shè)計(jì)通過EDC/NHS偶聯(lián)法實(shí)現(xiàn)了Ce6與DSPE-PEG 5000 -NH 2的共價(jià)結(jié)合。我們利用傅里葉變換紅外(FTIR)光譜法，

結(jié)論

綜上所述，我們成功構(gòu)建了用于成像引導(dǎo)和時(shí)空控制的光動(dòng)力* (PDT) 的 DSPE-PEG-Ce6 膠束。我們意外地發(fā)現(xiàn)，DSPE-PEG-Ce6 在細(xì)胞攝取后，以時(shí)間依賴性的方式依次與線粒體、溶酶體和內(nèi)質(zhì)網(wǎng)共定位。得益于 DSPE-PEG-Ce6 的動(dòng)態(tài)亞細(xì)胞分布和可調(diào)節(jié)的結(jié)合量，我們能夠仔細(xì)而準(zhǔn)確地評(píng)估位于線粒體、溶酶體和內(nèi)質(zhì)網(wǎng)的含 PS 納米劑的光動(dòng)力* (PDT) 療效。

Section snippets

Synthesis of DSPE-PEG-Ce6, methoxyl (OMe)-PEG5000-Ce6 (OMe-PEG-Ce6), and DSPE-PEG5000-fluorescein isothiocyanate (FITC) (DSPE-PEG-FITC)

To synthesize DSPE-PEG-Ce6, 1.60 mg Ce6 was dissolved in 1 mL dimethyl sulfoxide (DMSO). Then, 4.16 mg 1-ethyl-3-(3-(dimethyl-amino)propyl)carbodimide (EDC) and 3.09 mg N-hydroxysuccinimide (NHS) were further added into the above Ce6 solution for reaction at room temperature for 4 h. Next, the activated Ce6 was added to 5.17 mg DSPE-PEG5000-NH2 (predissolved in DMSO) for further reaction under stirring overnight. The mixture was dialyzed (molecular weight cut-off (MWCO) = 2 kDa) against DMSO

Synthesis and characterization of DSPE-PEG-Ce6 micelles

DSPE-PEG5000-NH2 was adopted as a drug carrier in our design because the long PEG chain can serve as a protective barrier against the biological components to prolong the circulation time of the encapsulated drug [30,31]. Ce6 is a second-generation PS and can be easily modified through its carboxyl groups for chemical conjugation. In our case, covalent binding of Ce6 to DSPE-PEG5000-NH2 was realized through the EDC/NHS coupling method. We used Fourier transform infrared (FTIR) spectroscopy,

Conclusions

In summary, we successfully fabricated DSPE-PEG-Ce6 micelles for imaging-guided and spatiotemporally-controlled PDT. We surprisingly found that DSPE-PEG-Ce6 colocalized with mitochondrion, lysosome, and ER successively after cellular uptake in a time-dependent manner. Benefiting from the dynamic subcellular distribution of DSPE-PEG-Ce6 with tunable binding amounts, we were able to carefully and accurately evaluate the PDT efficacies of the PS-containing nanoagents located at the mitochondrion,

西安pg電子官方生物提供相關(guān)產(chǎn)品：

YIGSR-PEG-DSPE

APRPG-PEG-DSPE

NGR-PEG-DSPE

NGR(c(GGCNGRC))-PEG-DSPE

RVG29-PEG-DSPE

THRPPMWSPVWP-PEG-DSPE

M2pep-PEG-DSPE

EB1-PEG-DSPE

CPP-PEG-DSPE

以上文章內(nèi)容來源各類期刊或文獻(xiàn)，如有侵權(quán)請(qǐng)聯(lián)系我們刪除！