文獻(xiàn)：Ultrafast Diffusion of a Fluorescent Cholesterol Analog in Compartmentalized Plasma Membranes

作者：Nao Hiramoto-Yamaki, Kenji A. K. Tanaka, Kenichi G. N. Suzuki, Koichiro M. Hirosawa, Manami S. H. Miyahara, Ziya Kalay, Koichiro Tanaka, Rinshi S. Kasai … See all authors 

文獻(xiàn)鏈接：https://onlinelibrary.wiley.com/doi/full/10.1111/tra.12163

Using the PtK2 cell line, which was previously employed by Eggeling et al. 22 and Sahl et al. 23, we examined the behaviors of Bdp-Chol and Cy3-DOPE in the top PM. Their typical trajectories are shown in Figure 1. A clearly remarkable feature of the trajectories in the intact cells, shown in Figure 1A, is that the tracking of a single Bdp-Chol molecule in the intact PM required an enhanced time resolution, from 33?milliseconds to 0.5?milliseconds, and that to place the typical trajectories of both Bdp-Chol and Cy3-DOPE in similar sizes, the trajectory lengths had to be different; i.e. 50- and 500-millisecond observation durations (at 0.5- and 33-millisecond resolutions for 100 and 15 steps, respectively). This clearly shows that Bdp-Chol diffused much faster than Cy3-DOPE.

我們研究了Bdp-Chol和Cy3-DOPE在頂部PM中的行為。

完整細(xì)胞中軌跡的一個(gè)明顯顯著特征是，在完整PM中追蹤單個(gè)Bdp-Chol分子需要提高時(shí)間分辨率，從33?毫秒到0.5?毫秒，為了使Bdp-Chol和Cy3-DOPE的軌跡大小相似，軌跡長度必須不同；即50毫秒和500毫秒的觀測持續(xù)時(shí)間（分別以0.5毫秒和33毫秒的分辨率進(jìn)行100步和15步）。這清楚地表明，Bdp-Chol的擴(kuò)散速度比Cy3-DOPE快得多。

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