表面上的聚合物-多肽/蛋白質(zhì)共聚物

本文概述了表面共價接枝的多肽/蛋白質(zhì)的合成方法及其物理、化學表征技術(shù)。獲得這種表面接枝的主要方法包括聚合物鏈的“嫁接到(grafting to)”或“嫁接于(grafting from)”方法，之后再附著肽/蛋白質(zhì)�；蛘呖梢詫�現(xiàn)成的合成物移植到表面上。

Abstract：

Background: Polymer-peptide/protein conjugates immobilized on surface have gained considerable attention, mostly due to their broad scope of applications in biomedical fields. To optimize their properties, the combined knowledge of polymer chemistry and biochemistry has to be applied.

Method: The main methods to obtain such surfaces involve “grafting to” or “grafting from” of polymeric linkers with subsequent attachment of a peptide/protein. Alternatively, a “ready-made” conjugate may be grafted to the surface.

Result: The structure of the polymer linker significantly influences the properties of the active surface, and a variety of physicochemical methods and tools have been successfully applied to study the structure-property relationship of these surfaces.

Conclusion: This review outlines the applied synthetic strategies leading to covalently attached bioconjugates on the surface and the characterization techniques used to study those materials. The relevance of different linear polymeric linkers and their influence on the properties of the obtained materials is discussed.