小分子交聯(lián)劑蛋白-蛋白以及蛋白-核酸大分子復(fù)合體材料

目前常用的小分子交聯(lián)劑主要與目標(biāo)蛋白Lys氨基酸殘基反應(yīng)，并且不具有時(shí)空分辨率。發(fā)展小分子交聯(lián)劑能夠特異性與多個(gè)氨基酸殘基進(jìn)行交聯(lián)并具有時(shí)空分辨率能夠促進(jìn)蛋白質(zhì)相互作用的研究。

帶有光保護(hù)的醌甲基化物（QM）小分子化學(xué)交聯(lián)劑。使用這些交聯(lián)劑，可以通過(guò)遠(yuǎn)紫外光（365 nm）誘導(dǎo)從而特異性的釋放活化的醌甲基化物進(jìn)行蛋白質(zhì)-蛋白質(zhì)以及蛋白質(zhì)-核酸之間的交聯(lián)。僅能夠使得蛋白交聯(lián)獲得時(shí)間分辨率，引入的強(qiáng)活性的醌甲基化物可與許多鄰近的親核性的殘基，如 His、Lys、Tyr 等側(cè)鏈反應(yīng)而產(chǎn)生蛋白交聯(lián)。并且這些交聯(lián)劑不僅使適用單個(gè)蛋白的交聯(lián)，也可以在細(xì)胞裂解液以及在活體細(xì)胞中誘導(dǎo)蛋白-蛋白發(fā)生化學(xué)交聯(lián)。

合成一端帶有醌甲基化物前體，另一端帶有NHS酯基的小分子化學(xué)交聯(lián)劑（NHQM）。通過(guò)對(duì)14-3-3 目標(biāo)蛋白紫外光照射與否以及加不加NHQM交聯(lián)劑處理，發(fā)現(xiàn)只有在加入NHQM交聯(lián)劑并且紫外光照射下才會(huì)誘導(dǎo)產(chǎn)生14-3-3二聚體。并且這種二聚體的產(chǎn)生可以很好的通過(guò)光控實(shí)時(shí)的產(chǎn)生。進(jìn)一步證明了NHQM交聯(lián)劑的其中一個(gè)實(shí)用性-可以快速鑒別蛋白聚合情況，并且利用化學(xué)交聯(lián)質(zhì)在14-3-3蛋白上與醌甲基化物反應(yīng)的親和性殘基。

接著，測(cè)試了NHQM在細(xì)胞內(nèi)的交聯(lián)情況，發(fā)現(xiàn)細(xì)胞體內(nèi)交聯(lián)效率較弱，這可能與短時(shí)間內(nèi)NHQM穿膜性和溶解性差有關(guān)，而增長(zhǎng)穿膜時(shí)間NHS會(huì)快速水解從而使交聯(lián)失效

pg電子官方供應(yīng)產(chǎn)品：

產(chǎn)品列表：

DHB

2,5-dihydroxybenzoic acid

CAS Number: 490-79-9

CHCA

α-Cyano-4-hydroxycinnamic acid

CAS Number: 28166-41-8

HABA

2-(4'-Hydroxybenzeneazo)benzoic acid

CAS Number: 1634-82-8

Sinapinic Acid  

CAS Number: 530-59-6

THAP

2',4',6'-Trihydroxyacetophenone monohydrate

CAS Number: 480-66-0

DHAP

2',4',-Dihydroxyacetophenone

CAS Number: 89-84-9

MBT

MBT MALDI matrix, 2-Mercaptobenzothiazole

CAS Number: 149-30-4

色譜相關(guān)試劑

產(chǎn)品列表：

LCMS Grade Solvents：

LCMS Acetonitrile     CAS Number: 75-05-8

LCMS Acetonitrile with 0.1% Acetic Acid

LCMS Acetonitrile with 0.1% Formic Acid

LCMS Acetonitrile with 0.1% TFA

LCMS Acetonitrile with 0.1% Formic Acid and 0.01% TFA

LCMS Water with 0.1% Acetic Acid

LCMS Water with 0.1% Formic Acid

LCMS Methanol       CAS Number: 67-56-1

LCMS Water          CAS Number: 7732-18-5

HPLC Grade Solvents：

HPLC Acetone           CAS Number: 67-64-1

HPLC Acetonitrile        CAS Number: 75-05-8

HPLC Acetonitrile with 0.1% TFA

HPLC Cyclohexane       CAS Number: 110-82-7

HPLC Dimethyl sulfoxide  CAS Number: 67-68-5

HPLC Methanol          CAS Number: 67-56-1

HPLC Water             CAS Number: 7732-18-5

Acetic Acid Ampules (Proteomics Grade)    CAS Number: 64-19-7

Formic Acid Ampules (Proteomics Grade)    CAS Number: 64-18-6

TFA Ampules (Proteomics Grade)          CAS Number: 76-05-1

HFBA Ampules (Proteomics Grade)         CAS Number: 375-22-4

Formic Acid + TFA Ampules (Proteomics Grade)

LCMS Mobile Phases Sampler Pack

Additives & Ion Pairing：

Proteochem蛋白交聯(lián)試劑，進(jìn)口蛋白交聯(lián)試劑

Protein crosslinkers （蛋白交聯(lián)相關(guān)試劑）：

Heterobifunctional Crosslinkers異型雙功能交聯(lián)劑：

SMCC Crosslinker

Succinimidyl-4-[N-maleimidomethyl]cyclohexane-1-carboxylate

CAS Number: 64987-85-5

Sulfo-SMCC Crosslinker

Sulfosuccinimidyl-4-[N-maleimidomethyl]cyclohexane-1-carboxylate

CAS Number: 92921-24-9

Sulfo-SANPAH Crosslinker

N-Sulfosuccinimidyl-6-[4'-azido-2'-nitrophenylamino] hexanoate

CAS Number: 102568-43-4

SMPH Crosslinker

Succinimidyl-6-[?-maleimidopropionamido]hexanoate

PDPH Crosslinker

3-[2-Pyridyldithio]propionyl hydrazide) is a cleavable, carbohydrate reactive

MBS Crosslinker

m-Maleimidobenzoyl-N-hydroxysuccinimide ester

CAS Number: 58626-38-3

Sulfo-MBS Crosslinker

m-Maleimidobenzoyl-N-hydroxysulfosuccinimide ester

SPDP Crosslinker

N-Succinimidyl 3-[2-pyridyldithio]-propionate

CAS Number: 68181-17-9

LC-SPDP Crosslinker  

Succinimidyl 6-(3-[2-pyridyldithio]-propionamido)hexanoate

Sulfo-LC-SPDP Crosslinker

Sulfosuccinimidyl 6-(3'-[2-pyridyldithio]-propionamido)hexanoate

SIA Crosslinker

N-Succinimidyl iodoacetate

CAS Number: 39028-27-8

SBA Crosslinker

N-Succinimidyl bromoacetate; SIB crosslinker

CAS Number: 42014-51-7

SMPB Crosslinker

N-Succinimidyl 4-[4-maleimidophenyl]butyrate

CAS Number: 79886-55-8

BMPS Crosslinker

N-(?-Maleimidopropyloxy)succinimide ester

CAS Number: 55750-62-4

EMCS Crosslinker

N-[e-Maleimidocaproyloxy]succinimide ester

CAS Number: 55750-63-5

GMBS Crosslinker

N-[Gamma-Maleimidobutyryloxy] Succinimide

CAS Number: 80307-12-6

ANB-NOS Crosslinker

N-5-Azido-2-nitrobenzoyloxysuccinimide

CAS Number: 60117-35-3

Homobifunctional Crosslinkers同型雙功能交聯(lián)劑：

BS3 Crosslinker

Bis[sulfosuccinimidyl] suberate

CAS Number: 82436-77-9

DSS Crosslinker

Disuccinimidyl suberate   N-hydroxysuccinimide ester

CAS Number: 68528-80-3

BS2G Crosslinker

BS2G; Bis[Sulfosuccinimidyl] glutarate

DSG Crosslinker

Disuccinimidyl glutarate; Di(N-succinimidyl

CAS Number: 79642-50-5

DSP Crosslinker

3,3'-Dithiodipropionic acid di(N-hydroxysuccinimide ester)

CAS Number: 57757-57-0

DTSSP Crosslinker

3,3?-Dithiobis[sulfosuccinimidylpropionate

CAS Number: 81069-02-5

Sulfo-EGS Crosslinker

glycolbis(sulfosuccinimidylsuccinate)

CAS Number: 167410-92-6

EGS Crosslinker

Ethylene glycolbis(succinimidylsuccinate)

CAS Number: 70539-42-3

DST Crosslinker  

CAS Number: 62069-75-4

Zero-Length Crosslinkers - EDC-HCl, Sulfo-NHS & NHS：

EDC-HCl

1- Ethyl-3-[3-dimethylaminopropyl]carbodiimide hydrochloride

CAS Number: 25952-53-8

Sulfo-NHS

N-hydroxysulfosuccinimide  Hydroxy-2,5-dioxopyrrolidine-3-sulfonicacid

CAS Number: 106627-54-7

進(jìn)口蛋白質(zhì)交聯(lián)試劑列表，Sulfo-LC-SPDP

Protein crosslinkers （蛋白交聯(lián)相關(guān)試劑）：

Heterobifunctional Crosslinkers異型雙功能交聯(lián)劑：

SMCC Crosslinker           CAS Number: 64987-85-5

Sulfo-SMCC Crosslinker      CAS Number: 92921-24-9

Sulfo-SANPAH Crosslinker    CAS Number: 102568-43-4

SMPH Crosslinker

PDPH Crosslinker

MBS Crosslinker             CAS Number: 58626-38-3

Sulfo-MBS Crosslinker

SPDP Crosslinker            CAS Number: 68181-17-9

LC-SPDP Crosslinker  

Sulfo-LC-SPDP Crosslinker

SIA Crosslinker              CAS Number: 39028-27-8

SBA Crosslinker             CAS Number: 42014-51-7

SMPB Crosslinker           CAS Number: 79886-55-8

BMPS Crosslinker           CAS Number: 55750-62-4

EMCS Crosslinker           CAS Number: 55750-63-5

GMBS Crosslinker          CAS Number: 80307-12-6

ANB-NOS Crosslinker        CAS Number: 60117-35-3

Homobifunctional Crosslinkers同型雙功能交聯(lián)劑：

BS3 Crosslinker           CAS Number: 82436-77-9

DSS Crosslinker           CAS Number: 68528-80-3

BS2G Crosslinker

DSG Crosslinker          CAS Number: 79642-50-5

DSP Crosslinker           CAS Number: 57757-57-0

DTSSP Crosslinker        CAS Number: 81069-02-5

Sulfo-EGS Crosslinker     CAS Number: 167410-92-6

EGS Crosslinker          CAS Number: 70539-42-3

DST Crosslinker          CAS Number: 62069-75-4

Zero-Length Crosslinkers - EDC-HCl, Sulfo-NHS & NHS：

EDC-HCl               CAS Number: 25952-53-8

Sulfo-NHS              CAS Number: 106627-54-7

Deuterated Crosslinker BS3-d4

Deuterated Crosslinker BS2G-d4

Deuterated Crosslinker DSS-d4

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