文獻(xiàn)：Non-Covalently Functionalized of Single-Walled Carbon Nanotubes by DSPE-PEG-PEI for SiRNA Delivery

文獻(xiàn)鏈接：https://xueshu.baidu.com/usercenter/paper/show?paperid=38060aa6b16da1fd532d375e0351a6b6&site=xueshu_se

作者：KS Siu，Y Zhang，X Zheng，J Koropatnick，WP Min

摘要：

The expression of a gene can be specifically downregulated by small interfering RNA (SiRNA). Modified carbon nanotubes (CNT) can be used to protect SiRNA and facilitate its entry into cells. Regardless of that, simple and efficient functionalization of CNT is lacking. Effective SiRNA delivery can be carried out using non-covalently functionalized CNT, where non-covalent (versus covalent) functionalization is simpler and more expeditious. Non-covalently functionalized single walled carbon nanotubes (SWCNT) that include a lipopolymer are described here. Polyethylenimine (PEI) conjugated to 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[amino(polyethylene glycol)-2000] (DSPE-PEG) was generated and the products used to disperse CNT to form DSPE-PEG-PEI/CNT (DGI/C), an agent capable of facilitating SiRNA delivery to cells in vitro and organs and cells in vivo.

基因的表達(dá)可以被小干擾RNA（SiRNA）特異性下調(diào)。改性碳納米管（CNT）可用于保護(hù)SiRNA并促進(jìn)其進(jìn)入細(xì)胞。盡管如此，仍缺乏簡單有效的CNT功能化。有效的SiRNA遞送可以使用非共價(jià)官能化的CNT進(jìn)行，其中非共價(jià)（與共價(jià)相比）官能化更簡單、更快捷。這里描述了包括脂聚合物的非共價(jià)官能化單壁碳納米管（SWCNT）。生成了與1,2-二硬脂酰基-sn-甘油-3-磷酸乙醇胺-N-[氨基（聚乙二醇）-2000]（DSPE-PEG）偶聯(lián)的聚乙烯亞胺（PEI），并將產(chǎn)物用于分散CNT以形成DSPE-PEG-PEI/CNT（DGI/C），這是一種能夠促進(jìn)SiRNA在體外遞送到細(xì)胞和體內(nèi)器官和細(xì)胞的試劑。

相關(guān)推薦產(chǎn)品：

DSPE-PEG-PEI

DSPE-PEG-PLA

DSPE-PEG-PLGA

DSPE-PEG-Progestrone

DSPE-PEG-RGD

DSPE-PEG-SC

DSPE-PEG-SCM

DSPE-PEG-SG

DSPE-PEG-SH

DSPE-PEG-Silane

DSPE-PEG-SP2-AA

版權(quán)聲明：

本平臺(tái)根據(jù)相關(guān)科技期刊文獻(xiàn)、教材以及網(wǎng)站編譯整理的內(nèi)容，僅用于對(duì)相關(guān)科學(xué)作品的介紹、評(píng)論以及課堂教學(xué)或科學(xué)研究。如有侵權(quán)，請(qǐng)聯(lián)系我們刪除。