文獻(xiàn)：HER2-specific affibody-conjugated thermosensitive liposomes (Affisomes) for improved delivery of anticancer agents.

文獻(xiàn)鏈接：https://xueshu.baidu.com/usercenter/paper/show?paperid=b7066b9e17eebe26a02523fad0cc64f8&site=xueshu_se

作者：A Puri，G Kramer-Marek，R Campbell-Massa，A Yavlovich，SC Tele，SB Lee，JD Clogston，AK Patri，R Blumenthal，J Capala

摘要：

The effects of this modification on physical characteristics and stability of the resulting nanoparticles denoted as Affisomes were investigated. Thermosensitive small unilamellar vesicle (SUV) liposomes of (80100 nm) a diameter consisting of dipalmitoyl phosphatidylcholine (DPPC, Tm 41°C) as the matrix lipid and a maleimide-conjugated pegylated phospholipid (DSPE-MaL-PEG2000) were prepared by probe sonication. Fluorescent probes were incorporated into liposomes for biophysical and/or biochemical analysis and/or triggered-release assays. Affibody was conjugated to these liposomes via its C-terminal cysteine by incubation in the presence of a reducing agent (e.g., tributylphosphine) for 1620 hours under an argon atmosphere. 

研究了這種修飾對(duì)所得納米顆粒（稱為仿射體）的物理特性和穩(wěn)定性的影響。通過(guò)探針超聲處理制備了直徑為（80100 nm）的熱敏小單層囊泡（SUV）脂質(zhì)體，由二棕櫚酰磷脂酰膽堿（DPPC，Tm 41°C）作為基質(zhì)脂質(zhì)和馬來(lái)酰亞胺偶聯(lián)的聚乙二醇化磷脂（DSPE-MaL-PEG2000）組成。熒光探針被摻入脂質(zhì)體中，用于生物物理和/或生化分析和/或觸發(fā)釋放分析。通過(guò)在還原劑（如三丁基膦）存在下在氬氣氣氛下孵育1620小時(shí)，將附著體通過(guò)其C-末端半胱氨酸與這些脂質(zhì)體結(jié)合。

相關(guān)推薦：

DSPE-PEG-SC

DSPE-PEG-SCM

DSPE-PEG-SG

DSPE-PEG-SH

DSPE-PEG-Silane

DSPE-PEG-SP2-AA

DSPE-PEG-SS

DSPE-PEG-TCO

DSPE-PEG-Tetrazine

DSPE-PEG-TMS

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