文獻(xiàn)：Development of PLGA-PEG-COOH and Gelatin-Based Microparticles Dual Delivery System and E-Beam Sterilization Effects for Controlled Release of BMP-2 and IGF-1

作者：Yan Bai, Seyedsina Moeinzadeh, Sungwoo Kim, Youngbum Park, Elaine Lui, Hua Tan, Weiling Zhao, Xiaobo Zhou, Yunzhi Peter Yang

文獻(xiàn)鏈接：https://onlinelibrary.wiley.com/doi/abs/10.1002/ppsc.202000180

摘要：

A poly(lactic-co-glycolic acid)-poly(ethylene glycol)-carboxyl (PLGA-PEG-COOH) and gelatin-based microparticles (MPs) dual delivery system for release of bone morphogenetic protein-2 (BMP-2) and insulin-like growth factor-1 (IGF-1) is developed. The delivery system is characterized based on its morphology, loading capacity, encapsulation efficiency, and release kinetics. The effects of electron beam (EB) sterilization on BMP-2 and IGF-1-loaded MPs and their biological effects are examined. The synergistic effect of a controlled dual release of BMP-2 and IGF-1 on osteogenesis of human mesenchymal stem cells (MSCs) is evaluated. Encapsulation efficiency of growth factors into gelatin and PLGA-PEG-COOH MPs is in the range of 64.78% to 76.11%. E-beam sterilized growth factor delivery systems are effective in significantly promoting osteogenesis of MSCs, although E-beam sterilization decreases the bioactivity of growth factors in MPs by ≈22%. BMP-2 release behavior from gelatin MPs/PEG hydrogel shows a faster release (52.7%) than that of IGF-1 from the PLGA-PEG-COOH MPs/PEG hydrogel (27.3%). The results demonstrate that the gelatin and PLGA-PEG-COOH MPs-based delivery system can realize temporal release of therapeutic biomolecules by incorporating different growth factors into distinct microparticles. EB sterilization is an accessible method for sterilizing growth factors-loaded carriers, which can pave the way for implementing growth factor delivery in clinical applications.

開發(fā)了一種用于釋放骨形態(tài)發(fā)生蛋白-2（BMP-2）和胰島素樣生長因子-1（IGF-1）的聚乳酸-聚乙二醇-羧基（PLGA-PEG-COOH）和明膠基微粒（MP）雙遞送系統(tǒng)。

遞送系統(tǒng)的特征在于其形態(tài)、負(fù)載能力、包封效率和釋放動(dòng)力學(xué)。研究了電子束（EB）滅菌對負(fù)載BMP-2和IGF-1的MP的影響及其生物學(xué)效應(yīng)。評估了BMP-2和IGF-1的受控雙重釋放對人間充質(zhì)干細(xì)胞（MSCs）成骨的協(xié)同作用。

生長因子在明膠和PLGA-PEG-COOH MP中的包封效率在64.78%至76.11%之間。電子束滅菌的生長因子遞送系統(tǒng)能有效顯著促進(jìn)MSCs的成骨，盡管電子束滅菌會(huì)使MP中生長因子的生物活性降低約22%。

明膠MPs/PEG水凝膠的BMP-2釋放行為比PLGA-PEG-COOH MPs/PEG凝膠的IGF-1釋放行為（27.3%）更快（52.7%）。結(jié)果表明，基于明膠和PLGA-PEG-COOH MP的遞送系統(tǒng)可以通過將不同的生長因子摻入不同的微粒中來實(shí)現(xiàn)治療性生物分子的暫時(shí)釋放。

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