多肽改性含糖共聚物電紡纖維

Abstract

Oligo(Glu70-co-Leu30), a peptide synthesized by protease catalysis, is functionalized at the N-terminus with a 4-pentenoyl unit and grafted to polyLSL[6′Ac,6″Ac], a glycopolymer prepared by ring-opening metathesis polymerization of lactonic sophorolipid diacetate. First, polyLSL[6'Ac,6”Ac] fiber mats are fabricated by electrospinning. Oxidation of the fiber mats and subsequent reaction with cysteamine lead to thiol-functionalized fiber mats with no significant morphology changes. Grafting of the alkene-modified oligopeptide to thiol-functionalized polyLSL[6′Ac,6″Ac] fiber mats is achieved via “thiol-ene” click reaction. X-ray photoelectron spectroscopy analysis to characterize peptide grafting reveals that about 50 mol% of polyLSL[6′Ac,6′′Ac] repeat units at fiber surfaces are decorated with a peptide moiety, out of which about 1/3 of the oligo(Glu70-co-Leu30) units are physically adsorbed to polyLSL[6′Ac,6′′Ac]. The results of this work pave the way to precise engineering of polyLSL fiber mats that can be decorated with a potentially wide range of molecules that tailor surface chemistry and biological properties.

本文介紹了聚LSL[6’Ac,6” Ac]電紡纖維的織造和固態(tài)表征過(guò)程，并記述了聚LSL[6’Ac,6” Ac]非織造氈與多肽的多種耦聯(lián)過(guò)程。X光光子光譜分析結(jié)果說(shuō)明，約表面50mol%的聚LSL[6’Ac,6” Ac]重復(fù)單元被多肽修飾，其中有1/3的多肽被聚LSL[6’Ac,6” Ac]物理吸附。